- #Snapgene viewer restriction enzyme digest how to
- #Snapgene viewer restriction enzyme digest install
- #Snapgene viewer restriction enzyme digest software
Select the enzymes you would like to use in your digestion reaction. Select single, double or triple cutting enzymes via the dropdown menu or use the search option to find specific enzymes. To get an overview of all the Restriction enzymes cutting in your molecule, click on Restriction enzymes. Also the format of the molecule could be changed to Linear or Plasmid. In the Settings the name of the molecule can be changed. Click on Blast and the sequence of the molecule is directly uploaded for a Blast.
To Blast your molecule there is a direct integration with the NCBI Blast. Not only the nucleotide sequence can be copied, also the Amino acid translation can be copied in the multiple frames.Įxport your molecule to Fast (.fasta), GenBank (.gb) or as an image by clicking on Export. Select the desired sequence or have the complete molecule copied. Click on Open reading frames and adjust how it is needed.Ĭopy your nucleotide sequence to use in any other program. See if there are other possible open reading frames in your molecule by changing the frame. If wanted the feature could be first selected in the sequence and this selection could be used.Ĭlick on Add new feature and the new feature will be present in both the linear view and circular view. Give it a name, choose the type of feature from the dropdown menu, give the start and end, and select the strand (forward or reverse). To add a new feature, click on + Add new feature. If wanted the color could be changed, and data could be reorganized by clicking on Name, Start, End, Strand, or Type. To get a list of all the features click on Features and all features available in the molecule will be displayed. To retrieve information of the feature, click on the feature and get the type, start and end-position, length, strand, GC content, TM and the start of the sequence. The important features, restriction enzymes, and open reading frames are directly visualized. Visualize your molecules in either a linear, circular, or combined view. Save your CloneAssist section by clicking on S ave.
fa), SnapGene (.dna) or CloneManager (.cm5) files. To add new molecules to CloneAssist Click on + and load new molecules. In CloneAssist you can visualize DNA molecules and the corresponding characteristics, annotate DNA molecules, perform digestion reactions, perform ligation reactions, and perform PCR reactions. Once the add-on has been installed, CloneAssist is available as a new section type to add to the ELN.Īfter creating the section, you can start a new CloneAssist project by clicking Start new.
#Snapgene viewer restriction enzyme digest install
To use CloneAssist, go to the Marketplace and install the CloneAssist add-on.
#Snapgene viewer restriction enzyme digest how to
Please contact us for more information on how to apply for the extended free trial. During this phase we offer a free extended 1-year trial. By installing CloneAssist you confirm that you understand the use of this add-on may cause irregularities and that eLabNext cannot be held responsible for any damage that this may cause.
#Snapgene viewer restriction enzyme digest software
With CloneAssist you can visualize DNA and plasmids and apply various molecular cloning techniques.ĬloneAssist is released as beta version and may contain bugs that create irregular behavior/glitches in the software or generate unexpected or incorrect results. The CloneAssist add-on offers a molecular cloning suite to support Molecular Biology R&D labs.
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